Composite

Part:BBa_K3431044:Design

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-10-17)


zr146_B_ToeholdSwitch-Regulated Invertase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
    Illegal NheI site found at 1421
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal BglII site found at 1192
    Illegal BamHI site found at 1322
    Illegal XhoI site found at 1393
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 40
    Illegal AgeI site found at 993
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We measure the thermodynamic characteristic of toehold switch and measure the glucose concentration with glucose meter to determine the efficacy of our composite device.


Source

This is a synthetic construct based on the work of Green et.al.

Green, A. A., Silver, P. A., Collins, J. J., & Yin, P. (2014). Toehold switches: de-novo-designed regulators of gene expression. Cell, 159(4), 925-939.

References